The Role of Periosteal Cell Mechanotrans-duction in Load-Induced Bone Formation
Abstract
The periosteum is a thin tissue surrounding bone that contains stem/progenitor cells involved in bone development, growth, repair, and load-induced bone formation. BMP signaling is critical for these biological processes. Here, we investigate the role of BMP signaling in appositional growth and mechanically induced osteogenic differentiation of periosteal cells. We designed an ex vivo appositional growth model and generated a periosteum-derived cell line to interrogate BMP2-mediated BMP signaling. Using these tools, we found that Bmp2 expression is upregulated in mechanically stimulated periosteal cells. When BMP2 is removed from periosteal lineage cells, BMP signaling is lost and appositional growth is severely attenuated. This work will enhance our understanding of periosteal activity and the role of BMP2 in load-induced bone formation.
Learning Objectives
1. How does BMP2 and associated BMP signaling regulate periosteal activity?
2. What is the role of BMP signaling in periosteal cell mechanotransduction?
3. Is the primary cilium important for periosteal cell BMP signaling?