Tissue engineering scaffolds increasingly seek to mimic complex biomolecular and mechanical gradients reflective of the tissue(s) of interest to guide regeneration, especially at complex tissue interfaces such as the osteochondral interface of the mandibular condyle.
As the ability of emerging additive manufacturing technologies to fabricate constructs presenting biomolecular and mechanical gradients continues to evolve, limited access to such cutting-edge technologies may dampen widespread application. Ko et al. report in a recent article a simple and efficient method of sequentially stacking photocrosslinkable prehydrogel solutions to generate hydrogel constructs with controlled biomolecular and mechanical gradients. Such an approach may enable development of hydrogel constructs presenting controlled gradients for a variety of tissue engineering applications, ranging from in vitro models for mechanistic study to scaffolds for condylar regeneration.
A simple layer-stacking technique to generate biomolecular and mechanical gradients in photocrosslinkable hydrogels.
Ko H, Suthiwanich K, Mary H, Zanganeh S, Hu SK, Ahadian S, Yang YP, Choi G, Fetah K, Niu Y, Mao J, Khademhosseini A. Biofabrication. 2019 Feb 20. doi: 10.1088/1758-5090/ab08b5. [Epub ahead of print]