Argon plasma modification promotes adipose derived stem cells osteogenic and chondrogenic differentiation on nanocomposite polyurethane scaffolds; implications for skeletal tissue engineering
University of Texas Health Science Center at Houston – School of Dentistry/Griffin MF, Ibrahim A, Seifalian AM, Butler PEM, Kalaskar DM, Ferretti P.
A successful scaffold for craniofacial tissue regeneration must enable appropriate interactions with cells to support tissue formation. In some cases the surface topography of a scaffold material can be tuned to mimic nanoscale features of the extracellular matrix and direct cell behavior. A recent article by Griffin et al. applied a simple gas plasma modification technique to modify the surfaces of polyurethane-based nanocomposite scaffolds for bone and cartilage tissue engineering. The authors completed plasma surface modification of the scaffolds using three common gases (argon, nitrogen, and oxygen) to investigate the effect of the gas used on the osteogenic and chondrogenic differentiation of adipose-derived stem cells in culture. Plasma treatment using argon was found to enhance chondrogenic and osteogenic differentiation of the cells to a greater extent than observed with nitrogen or oxygen. Moreover, the argon-treated scaffolds supported enhanced vascular tissue infiltration in a chick chorioallantoic membrane model. Overall, the article suggests that a simple argon surface modification technique can be applied to promote desired osteogenic differentiation of cells, which may benefit translational biomaterial-based approaches for craniofacial bone repair.
Griffin MF, Ibrahim A, Seifalian AM, Butler PEM, Kalaskar DM, Ferretti P. Mater Sci Eng C Mater Biol Appl. 2019;105:110085. doi: 10.1016/j.msec.2019.110085. [Epub ahead of print]