Bioactive glass-containing hydrogel delivery system for osteogenic differentiation of human dental pulp stem cells

Sevda P. Sevari  Faezeh Shahnazi  Chider Chen  John C. Mitchell  Sahar Ansari  Alireza Moshaverinia

Dental pulp-derived stem cells present osteogenic potential and have been explored for craniofacial bone tissue engineering applications, due in part to the relative ease with which they can be harvested. The utility of dental pulp-derived stem cells in tissue regeneration, however, requires an effective method of delivery to the defect site and an appropriate microenvironment to support osteogenesis. A recent article by Sevari et al. characterized a composite hydrogel system comprising an interpenetrating network of alginate and Matrigel laden with bioactive glass microparticles for the delivery of dental pulp-derived stem cells. The authors suggest that the hydrogel composite leverages the biological activity of Matrigel, the osteogenicity of bioactive glass microparticles, and the mechanical properties of alginate to create a suitable microenvironment to support osteogenesis of dental pulp-derived stem cells embedded within it. The authors report that dental pulp-derived stem cells remained viable within the hydrogels over the course of 14 days of culture, and the elasticity of the hydrogels decreased as the Matrigel content increased. Despite the decrease in the elasticity of the hydrogels, the presence of Matrigel was found to promote osteogenic differentiation (although it should be noted that the culture medium contained osteogenic supplements). The authors submit that the hydrogel composite may be a suitable delivery system for dental pulp-derived stem cells for craniofacial bone regeneration applications. While the study presents several limitations, it underscores the importance of biological and mechanical cues from the microenvironment of a hydrogel cell delivery vehicle on the phenotype of the encapsulated cells.

Citation Information:

Sevari SP, Shahnazi F, Chen C, Mitchell JC, Ansari S, Moshaverinia A. J Biomed Mater Res A. 2019. doi: 10.1002/jbm.a.36836.

PMID: 31709717

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