Human platelet lysate-based nanocomposite bioink for bioprinting hierarchical fibrillar structures
The emergence of bioprinting technologies in recent years presents exceptional promise to enable fabrication of patient-specific three-dimensional constructs for tissue engineering and regenerative medicine applications.
Given the geometric complexity of craniofacial structures, the potential of bioprinting technologies to advance craniofacial reconstruction cannot be overstated. However, progress in bioprinting has been limited by the complexity associated with the development of bioinks that are compliant with the requirements for 3D printing while being supportive of the viability of cells within them. A recent article by Mendes et al. applied human platelet lysate reinforced with cellulose nanocrystals in the development of a bioink for bioprinting. The authors sought to leverage the biological activity of the various growth factors and cytokines within platelet lysate to support cell viability and spreading in parallel with the fibrillar hierarchical structure of the hydrogel composite to mimic architectural features of the extracellular matrix. The report involves optimization of printing parameters and demonstrates successful printing of complex structures. The authors report viability, spreading, and proliferation of human adipose-derived stem cells within the bioprinted constructs in vitro, even in serum-free culture conditions. Moreover, the authors report remodeling of the bioprinted matrix by cells during culture. Overall, the article suggests the potential for platelet lysate-based composite bioink to enable fabrication of bioprinted constructs providing biologically active factors to promote cell viability and support matrix remodeling for tissue engineering applications.
Human platelet lysate-based nanocomposite bioink for bioprinting hierarchical fibrillar structures.
Mendes BB, Gomez-Florit M, Hamilton AG, Detamore MS, Domingues RMA, Reis RL, Gomes M. Biofabrication. 2019. doi: 10.1088/1758-5090/ab33e8. [Epub ahead of print]