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Marco C. Bottino, DDS, MSc, PhD, FADM

University of Michigan

Novel Bioceramic-based 3D Printed Hybrid Scaffold for Local MicroRNA Delivery

Marco C. Bottino, D.D.S., MSc., Ph.D., FADM Peter Geistlich Research Grant Winner


Dr. Marco Bottino is Associate Professor in the Department of Cariology, Restorative Sciences, and Endodontics at the University of Michigan. He received his dental degree from Universidade Paulista (São Paulo, Brazil), his master’s in Nuclear Technology from the University of São Paulo, and his doctorate in Materials Science from The University of Alabama at Birmingham. Marco has received Young InvestigatorAwards (Pulp Biology and Implantology Groups) from the International Association for Dental Research (IADR) and research grants from the NIH. Marco is President-elect of the Dental Materials Group of the IADR. His research interests are in the design of scaffolds and stem cell therapies to regenerate oral and craniofacial tissues.


Regeneration of alveolar bone remains a challenge, particularly in sites where vertical bone augmentation is needed for dental implant placement. With the US population aging, there is a critical need to find novel biomaterials and technologies to reliably promote the reconstruction of oral, cranial, and maxillofacial bone defects. Current regenerative strategies for alveolar bone defects using autogenous grafts are challenging due to the limited availability and donor site morbidity. On the other hand, pre-sized bone substitutes (synthetic and animal-derived) have suffered from the lack of shaping ability, poor mechanical competency, and variable degradation profile, thus potentially affecting the regenerative outcomes. 3D printing has become an attractive option to generating defect-specific and mechanically-strong biodegradable scaffolds for the reconstruction of oral, cranial and maxillofacial bone deficiencies. The proposed bioceramic ink incorporates highly degradable amorphous magnesium phosphate (AMP) particles as opposed to conventional crystalline calcium phosphates (e.g., hydroxyapatite, HAp). The hypothesis is that AMP particles can be used to formulate an innovative bioceramic ink, which ultimately will be used to fabricate defect-specific constructs with controlled degradation kinetics. Another unique aspect of the proposed work is that the AMP-based 3D printed macroporous scaffolds will be impregnated with a novel miRNA-modified hydrogel with the ability to regulate stem cell differentiation and angiogenesis. The aim of this study is to establish an innovative, cell- free biomaterials-based technology capable of generating via 3D printing defect-specifics constructs for oral and maxillofacial bone regeneration.


    Dubey N, Ferreira JA, Malda J, Bhaduri SB, Bottino MC. Extracellular Matrix/Amorphous Magnesium Phosphate Bioink for 3D Bioprinting of Craniomaxillofacial Bone Tissue. ACS Appl Mater Interfaces. 2020 May 27;12(21):23752-23763. doi: 10.1021/acsami.0c05311. Full Text

    Dubey N, Ferreira JA, Daghrery A, Aytac Z, Malda J, Bhaduri SB, Bottino MC. Highly tunable bioactive fiber-reinforced hydrogel for guided bone regeneration. Acta Biomater. 2020 Sep 1;113:164-176. doi: 10.1016/j.actbio.2020.06.011. Full Text

    Sikder P, Ferreira JA, Fakhrabadi EA, Kantorski KZ, Liberatore MW, Bottino MC, Bhaduri SB. Bioactive amorphous magnesium phosphate-polyetheretherketone composite filaments for 3D printing. Dent Mater. 2020 Jul;36(7):865-883. doi: 10.1016/ Full Text 

    Daghrery A, Ferreira JA, Xu J, Golafshan N, Kaigler D, Bhaduri SB, Malda J, Castilho M, Bottino MC. Tissue-specific melt electrowritten polymeric scaffolds for coordinated regeneration of soft and hard periodontal tissues. Bioact Mater. 2022 Apr 22;19:268-281. doi: 10.1016/j.bioactmat.2022.04.013 Full Text


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